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Measuring Molecular Mobility with Macro Fluorescence Imaging
18 Aug 2010: This application note examines GFP in live plants and observes systematic differences in areas non-invasively. This demonstrates that molecular mobility can be measured by macro imaging with steady state fluorescence anisotropy with the additional benefits of a large field-of-view which compliments both high throughput, in-vitro assay and in-vivo, non-invasive studies.
UVP, LLC.
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Trans-illumination – a Solution for Excitation of GFP Transfected Plants in Petri Dishes for In Vivo Imaging
18 Aug 2010: This application note shows that the excitation of GFP-transfected plants in Petri dishes filled with agar can be easily performed by Trans-illumination, with no reflection on the plastic material as it would happen with Epi-illumination. Background light intensities are in the range of 800-1000 RLU at 470 nm and 10% intensity setting of the transilluminator.
BERTHOLD TECHNOLOGIES GmbH & Co. KG
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Use of Antibodies In Small Animal Molecular Imaging
18 Aug 2010: In vivo imaging enables the natural course of a disease or experimental model to be monitored. This application note discusses the use of fluorescently labeled primary antibodies for looking at protein expression in vivo imaging. The use of antibodies allows researchers to quickly determine if the test antibody begins to target undesired organs such as the kidneys or livers.
Carestream Molecular Imaging
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Characterization of Ion Channels in Stem Cell-derived Cardiomyocytes on An Automated Parallel Patch Clamp System
22 Jun 2010: In this application note Molecular Devices use the PatchXpress 7000A automated parallel patch clamp system is used to record Na, K and Ca currents from stem cell-derived mouse cardiomyocytes. The collaborative effort demonstrates that the combination of “ready-to-use” cardiomyocytes with an automated patch clamp system offers a powerful assay platform for fast assessment of compound efficacy and for safety profiling of lead compounds in a biologically relevant system.
Molecular Devices
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Human Oncostatin M is Functionally Equivalent to Mouse LIF in Supporting Mouse ES Cell Pluripotency & Germline Competency
21 Jun 2010: In this application note R&D Systems demonstrate human OSM is able to maintain ES cell pluripotency in a similar manner to mouse LIF, including long-term maintenance of pluripotency and the ability to differentiate in vitro, the ability to activate STAT3, and germline transmission competency. The study suggests that OSM may be an additional factor that plays a role in early embryonic development and that it may be used as an alternative to LIF in mouse ES cell culture.
R&D Systems, Inc.
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Live Cell Imaging of Drug Effects on Golgi Morpholgy Using the CELLview™ Cell Culture Dish
01 Mar 2010: This application note by Greiner Bio-One shows that performing live-cell imaging experiments in parallel in CELLview™ dishes with four compartments allows a direct comparison of the speed and timing of drug effects on the Golgi apparatus. GalNAc-GFP cells can be easily imaged at multiple positions without cell death or focus problems.
Greiner Bio-One GmbH
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Automated Methods for Counting and Analysing Stem Cell Samples
09 Dec 2009: This application note by Nexcelom Bioscience describes automated methods for image-based cell counting, viability determination and fluorescence detection in stem cell samples. Concentration is automatically calculated helping to eliminate user to user variability. Unique brightfield/fluorescence imaging capabilities can be used to quantify transfection efficiency without having to use more complex methods, such as flow cytometry.
Nexcelom Bioscience
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SpectraPlex™ Western Blot Kit and the FluorChem® Q Provide a Complete Solution for Multicolor Fluorescent Western Blotting
03 Jul 2009: Traditional methods of Western blotting for sampling proteins rely on secondary antibodies and the blot is often stripped and re-probed for further proteins. This is time-consuming and can introduce artifacts to a sample. In this application article Alpha Innotech present the SpectraPlex kit and FluorChem Q Imaging system as a complete solution and suggest they provide superior sensitivity with faster imaging.
Cell Biosciences
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Development of a Novel Method to Assess Primary Hepatocyte Concentration and Viability
06 May 2009: Reliable concentration and viability data of primary Hepatocytes is critical for analysis of compound toxicity in vitro. Traditional manual counting methods are time consuming and subjectivity can cause inconsistent results. Nexcelom’s method incorporates fluorescent dual staining with a disposable counting chamber. Images cells are captured and Cellometer Vision’s software analyzes images; generating live cell count, concentration & viability percentage, typically in less than 60s.
Nexcelom Bioscience
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